least squares procedure in jmp pro 14.0 Search Results


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FUJIFILM 14:0/14:0-phosphatidylethanolamine (ptdetn (28:0
Flow chart illustrating sphingolipid, glycerophospholipid (GPL) and diacylglycerol (DAG) sample preparation and analysis. Butanol and ethyl acetate/hexane (1:1) were used for the first and second extractions, respectively. To reduce the ion suppression effects associated with large amounts of phosphatidylcholine (PtdCho) and <t>phosphatidylethanolamine</t> (PtdEtn) in the sample, a DEAE fractionation step was introduced for the preparation of acidic lipids. Depending on the analyte assayed, four different MS methods (I–IV) were used for higher sensitivity. Further details are available in the Materials and Methods Section. SRM, selected reaction monitoring; Sph, sphingosine; Cer, ceramide; HexCer, monohexosylceramide; SM, sphingomyelin; PtdSer, phosphatidylserine; S1P, sphingosine-1-phosphate; Cer1P, ceramide-1-phosphate; PtdOH, phosphatidic acid; PtdGro, phosphatidylglycerol; PtdIns, phosphatidylinositol; GM3, monosialoganglioside; STD, standard solution; FA, formic acid.
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Flow chart illustrating sphingolipid, glycerophospholipid (GPL) and diacylglycerol (DAG) sample preparation and analysis. Butanol and ethyl acetate/hexane (1:1) were used for the first and second extractions, respectively. To reduce the ion suppression effects associated with large amounts of phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) in the sample, a DEAE fractionation step was introduced for the preparation of acidic lipids. Depending on the analyte assayed, four different MS methods (I–IV) were used for higher sensitivity. Further details are available in the Materials and Methods Section. SRM, selected reaction monitoring; Sph, sphingosine; Cer, ceramide; HexCer, monohexosylceramide; SM, sphingomyelin; PtdSer, phosphatidylserine; S1P, sphingosine-1-phosphate; Cer1P, ceramide-1-phosphate; PtdOH, phosphatidic acid; PtdGro, phosphatidylglycerol; PtdIns, phosphatidylinositol; GM3, monosialoganglioside; STD, standard solution; FA, formic acid.

Journal: Metabolites

Article Title: Comparative Analysis of Biological Sphingolipids with Glycerophospholipids and Diacylglycerol by LC-MS/MS

doi: 10.3390/metabo4010098

Figure Lengend Snippet: Flow chart illustrating sphingolipid, glycerophospholipid (GPL) and diacylglycerol (DAG) sample preparation and analysis. Butanol and ethyl acetate/hexane (1:1) were used for the first and second extractions, respectively. To reduce the ion suppression effects associated with large amounts of phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) in the sample, a DEAE fractionation step was introduced for the preparation of acidic lipids. Depending on the analyte assayed, four different MS methods (I–IV) were used for higher sensitivity. Further details are available in the Materials and Methods Section. SRM, selected reaction monitoring; Sph, sphingosine; Cer, ceramide; HexCer, monohexosylceramide; SM, sphingomyelin; PtdSer, phosphatidylserine; S1P, sphingosine-1-phosphate; Cer1P, ceramide-1-phosphate; PtdOH, phosphatidic acid; PtdGro, phosphatidylglycerol; PtdIns, phosphatidylinositol; GM3, monosialoganglioside; STD, standard solution; FA, formic acid.

Article Snippet: The 14:0/14:0-phosphatidylethanolamine (PtdEtn (28:0)) and triheptadecanoin (TAG (17:0/17:0/17:0)) were obtained from Wako Pure Chemicals (Osaka, Japan).

Techniques: Sample Prep, Fractionation